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Each subsample was treated with 1M HCL (80° C for 2h), 1M Na OH (80° C for 2 h) and again in acid, with rinsing in between.

Two of the three samples were then bleached in Na OCL (2.5% at p H-3 for 30 min).

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Also, at two laboratories (Oxford and Zurich), after combustion to gas, the samples were recoded so that the staff making the measurements did not know the identity of the samples. On the basis of the Islamic embroidered pattern and Christian ink inscription, this linen could be dated to the eleventh to twelfth centuries AD. Linen from the collection of the Department of Egyptian Antiquities at the British Museum, associated with an early second century AD mummy of Cleopatra from Thebes (EA6707).

Even for the first investigation, there was a possibility of using radiocarbon dating to determine the age of the linen from which the shroud was woven.

The size of the sample then required, however, was ~500cm, which would clearly have resulted in an unacceptable amount of damage, and it was not until the development in the 1970s of small gas-counters and accelerator-mass-spectrometry techniques (AMS), requiring samples of only a few square centimetres, that radiocarbon dating of the shroud became a real possibility. The shroud was separated from the backing cloth along its bottom left-hand edge and a strip (~10 mm x 70 mm) was cut from just above the place where a sample was previously removed in 1973 for examination.

The Arizona group split each sample into four subsamples.

One pair of subsamples from each textile was treated with dilute HCL, dilute Na OH and again in acid, with rinsing in between (method a).